AviTag is a short peptide sequence commonly used in biotechnology and protein research for specific biotinylation. It’s typically composed of 15 amino acids (GLNDIFEAQKIEWHE) and is recognized by the biotin ligase enzyme (BirA), which attaches biotin to a specific lysine residue within the tag. This site-specific biotinylation allows researchers to label proteins in a controlled and predictable manner, making AviTag a valuable tool in protein purification, detection, and immobilization applications.
Due to its high specificity and efficiency, AviTag is widely used in fields such as drug discovery, diagnostic development, and therapeutic protein engineering.
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